【摘要】 目的 :分析长波紫外线照射诱发人皮肤成纤维细胞凋亡及活性氧生成的作用。方法 :用长波紫外线照射培养的人皮肤成纤维细胞 ,经流式细胞仪和荧光显微镜检测细胞凋亡情况 ,荧光分光光度计检测活性氧 ,电子自旋共振波谱仪检测自由基。结果 :110～ 12 0kJ·m-2 的UVA照射能够诱导明显的细胞凋亡 ,甘露醇能够拮抗这种作用 ,照射样品中发现活性氧生成增加 ,并检测到了羟自由基信号。结论 :低辐照度UVA(0 .6mW·cm-2 )照射能够诱发人皮肤成纤维细胞凋亡 ,氧化损伤可能在其中发挥着一定作用。更多还原

【Abstract】 Objective: To analyse the induction of apoptosis and reactive oxygen species (ROS) in human skin fibroblast by ultraviolet A (UVA). Methods: Human skin fibroblasts were irradiated with UVA, apoptosis was determined with flowcytometry and fluorescent microscopy, the intracellular ROS production was measured with a spectrofluorometer,and the free radicals were determined with electron spin resonance. Results: A significant increase of apoptosis was observed in human skin fibroblast after exposure to UVA with a dosage at 110-120 kJ·m -2 , mannitol could counteract apoptosis; a higher level of intracellular ROS and the hydroxyl free radical was detected in the irradiated samples. Conclusion: A lower dose of irradiation of UVA(0.6 mW·cm -2 ) can induce apoptosis in human skin fibroblast, and ROS might play a role in the induction of apoptosis.更多还原