【摘要】 目的 :探讨乙肝病毒 (HBV) C区基因变异与干扰素疗效的相互关系。方法 :采用荧光定量 PCR法对 30例经干扰素治疗的慢性乙肝患者治疗前后病毒滴度进行检测。选取有反应、复发、无反应的患者各 3例 ,进行基因序列分析。结果 :干扰素治疗的总有反应率为 2 6 .7% (8/ 30 ) ,C区的基因变异多发生于抗原表位附近 ,主要为点突变及缺失突变 ,多不引起氨基酸水平的改变。CTL 表位氨基酸相对保守 ,总变异率约为 9氨基酸 / 9人 ,而 Th表位氨基酸变异相对较多 ,总变异率为 5 5氨基酸 / 9人。结论 :干扰素治疗后 HBV C区核苷酸及编码的氨基酸突变率增加 ,且多集中于 C区抗原表位 ,CTL 表位变异可能与干扰素疗效有关。更多还原

【Abstract】 Objective:To study the relationship between hepatitis B virus core mutant and the effect of Interferon.Methods:The serum hepatitis B virus core mutant was detected by the method of fluorescence quantitative PCR in 30 chronic hepatitis B patients,and several patient’s serum were given gene sequence analysis.Results:The efficiency rate was 26.7%(7/30)in Interferon therapy patients,the mutants happened in the epitopes close to antigen.The CTL epitopes mutation rate was 9 aa./9 patients,but THC epitopes mutation rate was 55aa./9 patients. Conclusion:The results showed that antiviral therapy with Interferon can induce the hepatitis virus core mutant,and CTL epitopes mutation in the core region,especially,THC epitopes mutation was associated with INF treatment effect.更多还原