【摘要】 目的 :克隆人野生型抑癌基因 PTEN/MMAC1 c DNA序列并构建其表达载体。方法 :利用RT- nested PCR法从正常人胎盘组织中扩增出约 1 2 0 0 bp的 DNA片段 ,与 p UCm- T载体连接 ,作全自动测序确证 ,并将其重组入 pc DNA 3.1载体中 ,构建为表达质粒 pc DNA- w P。结果 :对 PCR产物进行测序 ,序列基本正确。结论 :利用 RT- nested PCR法成功克隆了人野生型抑癌基因 PTEN/MMAC1c DNA序列并构建了表达质粒 pc DNA- w P。更多还原

【Abstract】 Objective: To clone the cDNA of the human tumor suppressor gene PTEN/MMAC1 and construct its expression vector. Methods: About 1 200 bp DNA fragment was amplified from human placenta tissues by using RT nested PCR and was cloned into pUCm T vector after automatic sequenced, then the fragment was linked with expression vector pcDNA 3.1. Results:The PTEN/MMAC1 cDNA had been cloned correctly and its expression vector pcDNA wP also had been constructed. Conclusion: Human tumor suppressor gene-PTEN/MMAC1 cDNA had been cloned by RT nested PCR and its expression vector had been constructed successfully. The result makes the base for further study on the tumor suppression function of PTEN/MMAC1.更多还原