【摘要】 目的　了解呼吸道合胞病毒 (RSV)的亚型感染情况及其所致毛细支气管炎的临床特点。方法　对12 1例临床诊断为毛细支气管炎患儿的鼻咽分泌物采用巢氏PCR方法进行RSV及亚型检测 ,并分析亚型感染患儿的临床资料。结果　①RSV阳性 87例 (71.9% ) ,其中A亚型 6 0例 ,B亚型 2 7例 ;②Bierman’s病情评分显示B亚型毛细支气管炎的临床症状较A亚型轻 ,恢复快 ;③A、B亚型RSV感染毛细支气管炎患儿在发病年龄、发热、血气、胸片上无显著性差异 ;④与间接免疫荧光等方法相比较 ,巢氏PCR是检测RSV灵敏度较高的方法。结论　B亚型RSV毛细支气管炎的临床症状较A亚型为轻更多还原

【Abstract】 Objective To understand the clinical characteristics of bronchiolitis caused by respiratory syncytial virus (RSV) subgroup.Methods One hundred and twenty one specimens of nasopharyngeal aspirates from infants with bronchiolitis admitted to the Affiliated Children’s Hospital of the Capital Institute of Pediatrics during the period of the January of 1999 through the April of 2002 were detected for virus and subtype with nested PCR method.The clinical data of the patient diagnosed etiologically as RSV subgroup infection were analyzed.Results Eighty seven RSV were isolated with nested PCR,and the positive rate was 71.9%,sixty isolates were identified as RSV subtype A,twenty seven were subtype B.No differences were found in age,sex,fever,gas,radiological findings and WBC.Statistically significant differences existed in clinical manifestation and recovery between subtype A and B.Nested PCR is the most sensitive method for detection of RSV from clinical specimens.Conclusions The main subtype of RSV that caused bronchiolitis was subtype A.The clinical manifestation caused by subtype B was mild.更多还原