【摘要】 对用于猪体外受精 (IVF)的研究方法和技术 ,如传统的液滴IVF、透明带下注射精子受精 (SUZI)、卵母细胞质内单精注射受精 (ICSI)及细管IVF等进行了简述。与其它动物相比 ,进行猪卵的体外受精研究 ,多精受精现象特别明显。大量的研究表明 ,猪卵的多精受精不但与其品种特性有关 ,而且与卵母细胞成熟的程度、透明带的异常、受精时获能精子的浓度、输卵管分泌物、受精液蛋白添加成分、NaHCO3 浓度、咖啡因、pH值以及温度等因素密切相关。更多还原

【Abstract】 The methods and techniques used for porcine oocyte in vitro fertilization (IVF) are discussed. There are several methods of IVF used for porcine or other mammalian oocyte fertilization, such as conventional IVF in drops, sub-zonal injection (SUZI), intracytoplasmic sperm injection (ICSI) and straw IVF. There is a greater incidence of polyspermy in porcine oocytes compared to that in other mammalian oocytes. It was verified that a lot of factors affect the incidence of porcine oocyte polyspermy, including immature or aged oocytes, zona abnormality, the number of capacitated sperm at the site of fertilization, oviduct secretions, protein supplementation, pH, concentration of NaHCO-3 in the fertilization medium and the temperature of the oocyte-sperm co-culture and its species-specificity.更多还原