【摘要】 血管生长抑素是一种新的抑制血管生成的因子 ,它能够特异性地抑制血管生成。本文通过宫角注射法对血管生长抑素抑制小鼠胚胎植入的最低有效量进行了探讨 ,并通过明胶酶谱分析和免疫印迹等方法研究了不同剂量血管生长抑素对MMP 2和MMP 9活性及蛋白表达的影响。实验表明 ,宫角注射 4μg血管生长抑素是抑制小鼠胚胎植入的最低有效剂量 ,这一最低有效剂量显著抑制了小鼠子宫MMP 2和MMP 9的活性及蛋白的表达.更多还原

【Abstract】 Angiostatin, an internal proteolytic 38 kD cleavage product of plasminogen, has been shown to inhibit the process of angiogenesis or neovascularization. In the present study, we detected the minimum effective dose for angiostatin to inhibit embryo implantation using intra-uterine horn injection in the mouse, and analyzed the effect of angiostatin on MMPs activity and protein expression using gelatin zymography and Western blot. Our results show that different doses of angiostatin have different roles in inhibiting the number of implanted embryos. There was no significant decrease after 3 μg angiostatin treatment(P>0.05). Implanted embryos were significantly inhibited by 4 μg angiostatin at day 3(day 1=1st day on which vaginal plug observed)(P<0.05), and 5 μg or 6 μg angiostatin on day 4 or day 5. Both 5 μg and 6 μg angiostatin remarkably inhibited implanted embryos at day 3(P<0.05). We conclude that 4 μg is the minimum effective dose of angiostatin to inhibit embryo implantation at day 3 in the mouse. At the same time, we also detected the activity and protein expression of MMP-2 and MMP-9 in mouse uteri after different doses of angiostatin. Our results showed that 3 μg angiostatin had no effect on MMP-2 and MMP-9 activity and protein expression(P>0.05). But 4 μg, 5 μg or 6 μg angiostatin treatment significantly inhibited MMPs activity and expression at day 3(P<0.05). In summary, angiostatin might be an effective drug for inhibiting embryo implantation by inhibiting MMPs activity and blocking angiogenesis in the mouse uterus.更多还原