In order to obtain high-l evel secreted expression of hepati tis B virus(HBV) preS2+S gene in P ichia Pastoris system,the HBV surf ace antigen(HBsAg) preS2+S gene wa s cloned into the secreted express ion vector pPIC9K at first.The rec ombinant plasmid p9Kadw2S was then electroporoted into yeast Pichia P astoris cells KM71.Multiinsertion transformants were screened with G 418 resistance.After methanol indu ction,the expressed HBsAg protein were analyzed by SDS-PAGE and ELIS A.The high-level expression cl...
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