【摘要】 ①目的　探讨扇贝多肽 (PCF)对紫外线损伤Hela细胞的影响。②方法　培养 2 4h的Hela细胞 ,制备细胞悬液 (5× 1 0 8/L) ,分别置 2 4孔板 ,每孔 1mL。随机分为对照组 ,紫外线损伤模型组 ,5 g/LPCF组 ,1 0 g/LPCF组 ,2 0g/LPCF组和 1 0 g/L维生素C组。每组 3个复孔 ,分别在RPMI 1 6 4 0、PCF、维生素C孵育 1 0min后 ,分别置紫外线下照射 ,照射强度为UVA 36 5 0 μJ/cm2 、UVB 7.1 5× 1 0 -5J/cm2 。用流式细胞仪测定各组细胞的死亡率、凋亡率及细胞内Ca2 + 含量。用生物化学方法测定上清液中MDA含量和抗氧化酶的活性。③结果PCF可以降低紫外线损伤Hela细胞的死亡率和凋亡率 ,并且提高细胞内Ca2 + 含量和琥珀酸脱氧酶的活性。PCF还可降低MDA的含量 ,提高抗氧化酶GSH px、SOD和CAT的活性。 ④结论　PCF可能通过提高抗氧化酶的活性而发挥对紫外线损伤Hela细胞的保护作用更多还原

【Abstract】 Objective\ The investigate the effect of polypeptide isolated from Chlamys Farreri (PCF) on Hela cells damaged by ultraviolet(UV).\ Methods\ The suspension of Hela epithelia cells (5×10 8/mL ) cultured for 24 hours were prepared and 1 mL of it was put into each hole of 24 hole plates. Some Hela cells were randomly divided into six groups: control group (C), modal group(M), 5 g/L PCF group, 10 g/L PCF group, 20 g/L PCF group and 10 g/L Vitamin C (Vc) group. After incubation with RPMI 1640, PCF, Vitamin C, respectively, for 10 minutes, the cells were exposed to UVA ( irradiation intensity: 3 650 μJ/cm 2), with the exception of the mice in C group. Some other Hela cells were divided into another six groups, they were treated almost the same as above, but irradiated by UVB ( irradiation intensity: 7.15×10 -5 J/cm 2). The intracellular free calcium (Ca 2+ ), apoptosis and cell death rate were measured by flow cytometry (FCM). Enzyme activities and malondialdehyde (MDA ) were determined by biochemical assays.\ Results\ The apoptosis and cell death rate were decreased and the contents of intracellular Ca 2+ and activities of succinate dehydrogenase (SDH) as well as the activities of GSH px, SOD,CAT increased and the MDA decreased by the action of PCF. \ Conclusion\ It is suggested that PCF could protect the cells from the damage caused by UVA and UVB by enhancing the activities of antioxidant enzymes.更多还原