【摘要】 根据转Bt基因棉"国抗1号"转入的外源基因中整合有GUS基因和NPT 基因,建立了3种种子纯度检测方法:GUS组织化学染色法、NPT 培养基法和NPT 叶片涂抹法,准确率都达到98%以上;在检测转Bt基因棉原始群系"20-1"时与生物测定比较准确率也在98%以上,因此这3种方法可用于转Bt基因棉大规模制种过程中和推广过程中良种纯度的检测,相应方法同样适用于基因组中整合有GUS基因和(或)NPT 基因的其它转Bt基因棉的品种。更多还原

【Abstract】 According to GUS gene and NPTⅡgene transformed into Bt transgenic cotton cultivar "Guokang mian No1",three methods for testing the purity of the cotton seeds were established.The results indicated that the accurate rates were up to 98%,and the accurate rates were also up to 98% (compared to the result of bioassay) in testing the original Bt transgenic cotton group "201".The corresponding methods were suitable to every kind of Bt transgenic cotton cultivar with GUS gene or (and) NPTⅡgene.更多还原