【摘要】 背景与目的:研究表明肿瘤细胞周期分析显示细胞具高增殖率的肿瘤,其临床病情发展快;细胞周期调节蛋白Ki-67、细胞周期蛋白A及p27介入到细胞的增殖,但这些因子与食管癌之间的关系的研究在我国尚未见报道。本研究观察细胞周期调节蛋白Ki-67、细胞周期蛋白A及p27在中国食管癌患者中的表达特征,以探讨这些分子标志物与临床病理因素之间的关系。方法:60例(48例男性、12例女性)食管鳞癌患者行外科手术切除肿瘤,其标本行免疫组织化学染色。Ki-67及细胞周期蛋白A表达程度以染色指数表示,p27以标记指数表示。结果:Ki-67、细胞周期蛋白A及p27免疫组化染色在瘤组织与非瘤组织中均固定于细胞核。Ki-67及细胞周期蛋白A的染色指数在低分化鳞癌(27.2±4.9;15.4±5.3)明显高于高分化鳞癌(20.6±6.3;11.3±6.4,P<0.05);p27免疫组化染色的阳性率在高分化鳞癌(36%)高于其它病理类型(29%及18%),但相互之间的差异无统计学意义(P>0.05)。结论:Ki-67、细胞周期蛋白A及p27的表达程度可反映食管鳞癌细胞的增殖状况。细胞周期调节蛋白Ki-67及细胞周期蛋白A的过度表达提示食管鳞癌细胞分化差。更多还原

【Abstract】 BACKGROUND &OBJECTIVE: Tumor cell cycle analysis has indicated that tumors with a higher proliferation rate showed more aggressive clinical behavior. Cell cycle regulatory proteins Ki 67, cyclin A, and p27 are associated with cell proliferation. The objective of this study was to observe the character of expression of cell cycle regulatory proteins Ki 67, cyclin A, and p27 in esophageal carcinoma and to clarify the relationship between clinicopathologic and these proteins. METHODS: Immunohistochemistry for Ki 67, cyclin A, and p27 were carried out. Ki 67 and cyclin A staining index (SI), and p27 labeling index were examined and detailed pathologic examinations were conducted on tumors from 60 patients (48 males and 12 females with surgically resected esophageal squamous cell carcinoma). RESULTS: Ki 67, cyclin A, and p27 immunostaining were all confined to the nuclei in both neoplastic and non neoplastic cells. The staining indexes(SIs) of Ki 67 and cyclin A, were significantly higher in poorly differentiated squamous cell carcinoma (SCC,27.2±4.9;15.4±5.3) than in well differentiated SCC(20.6±6.3;11.3±6.4,P< 0.05). The p27 positive immunostaining in the nuclei in well differentiated SCC(36%)were higher than that in the other tumor types (29%;18%), but there was no significance(P< 0.25,P >0.05). CONCLUSION: The expression levels of Ki 67, cyclin A, and p27 may suggest the proliferative activity of cancer cells in Chinese patient with SCC of esophagus. The cell cycle regulatory proteins Ki 67 and cyclin A over expression correlates with poor SCC differentiation in patients with esophageal carcinoma.更多还原