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HPLC测定爽咽茶中绿原酸的含量
Determination of Chiorogenic Acid in Shuangyan Tea by HPLC
【摘要】 目的 :建立爽咽茶中绿原酸含量的测定方法。方法 :采用HPLC测定爽咽茶中绿原酸的含量 ,采用C1 8柱 ,乙腈 4 .0g L磷酸 (1 0∶90 )为流动相 ,检测波长为 32 7nm。结果 :平均加样回收率为 97.75 % ,RSD为 1 .4 0 %。结论 :本法操作简便、易行 ,具有实用性。
【Abstract】 Objective:To establish the determination method of chiorogenic acid in Shuangyan Tea. Method: The high performance liquid chromatography method was developed for the determination of chiorogenic acid in Shuangyan Tea on C 18 column in acetonitrile 4.0 g/L phosphoric acid(10∶90) as a mobile phase and detection wavelength at 327 nm. Result: The average recovery was 97.75% and RSD was 1.40%. Conclusion: The method is simple, quick, accurate,and has good reproducibility.
【关键词】 爽咽茶;
绿原酸;
高效液相色谱法;
【Key words】 Shuangyan Tea; Chiorogenic acid; High performance liquid chromatography;
【Key words】 Shuangyan Tea; Chiorogenic acid; High performance liquid chromatography;
- 【文献出处】 安徽中医学院学报 ,Journal of Anhui Traditional Chinese Medical College , 编辑部邮箱 ,2003年06期
- 【分类号】R286.0
- 【下载频次】50