【摘要】 目的　探讨核转录因子 -κB (NF -κB)与大鼠肝纤维化的关系。方法　用四氯化碳 (CCL4)制备大鼠肝纤维化模型 ,同时以能够抑制NF -κB激活的N -乙酰 -L -半胱氨酸 (NAC) ( 10 0mg/kg ,ip)作为对照。在造模的第 12周末处死大鼠 ,观察大鼠肝脏NF -κBP65的表达 ,测定血清丙氨酸转氨酶 (ALT)、天冬氨酸转氨酶 (AST )、白蛋白(ALB)水平及ALB与球蛋白 (GLO)比值 ,并进行肝脏病理学检查。结果　模型组大鼠肝脏表达NF -κB亚单位P65较正常大鼠明显增强 ,ALT、AST水平增高 ,ALB和ALB/GLO下降 ,肝脏炎症和纤维化评分显著升高 (P <0 .0 5～ 0 .0 1)。NAC则抑制NF -κB激活 ,降低了ALT、AST ,提高了ALB和ALB/GLO ,肝脏炎症和纤维化明显减轻 (P <0 .0 5～0 .0 1)。结论　NF -κB参与了大鼠肝纤维化过程 ,具有促炎、促纤维化作用更多还原

【Abstract】 Objective To evaluate the role of NF-κB inrat liver fibrosis. Methods Liver fibrosis in rats wereinduced by carbon tetrachloride (CCl 4), meanwhile, N-acetyl-L-cystein at the dose of 100 mg/kg body weight which could inhibit activity of NF-κB was treatedby i.p. as a control group. The rats were killed at the end of the 12 th week,and the expression of NF-κB subunit P65 in rat’ liver was analyzed, the levels of alanine transaminase (ALT), asparagic acid transaminase (AST) and albumin (ALB) in serum were measured and the ratio of albumin against globulin (GLO) wascalculated. Histological examination of rat liver was also measued. Results The expression of NF-κB subunit P65 in the liver of model group was stronger than that in normal rats. The levels of ALT, AST were increased, while ALB and ALB/GLO decreased, and the score of hepatic inflammation and liver fibrosis increased significantly ( P <0.05～0.01). When NAC inhibited theactivation NF-κB, the levels of ALT, AST decreased, ALB and ALB/GLO increased, hepatic inflammation and liver fibrosis in model rats improved obviously ( P <0.05～0.01). Conclusion NF-κB takes part in the course of rat liver fibrosis, and can promot the liver inflammation and fibrosis inrats.更多还原