【摘要】 选用宽叶刺槐带腋芽的茎段进行组织培养 ,结果表明 :适宜的诱导培养基是MS +6-BA 0 .3mg/L(单位下同 ) +NAA 0 .1;分化、增殖培养基MS +6-BA 0 .6+NAA 0 .2 ;生根培养基 1/2MS +IBA 0 .5 +NAA 0 .5 +AC 5 0 0。试管苗在自然光下封口炼苗 14d ,开瓶炼苗 2～ 3d ,移栽成活率达 90 %以上。更多还原

【Abstract】 Robinia pseudoacacia stem tissue culture was studied. The results showed that the best medium for inducement was MS+6-BA 0.3?mg/L+NAA 0.1?mg/L,and for multiplication was MS+6-BA?0.6?mg/L+NAA?0.2?mg/L,and for rooting culture was 1/2MS+IBA?0.5?mg/L+NAA?0.5?mg/L+AC?500?mg/L.The plantlets inside bottle had been sealed for two weeks and the plantlets, training, the bottle was opened for 2 or 3 days.The survival rate of transplanting plantlets was over 90%.更多还原