【摘要】 目的 :观察大蒜提取物(garlicextract,GE)和维生素E在拮抗烹饪油烟凝集物(condensatesofcookingoilfume,COF)的致突变的作用。 方法 :在人外周血淋巴细胞培养物中注入一定剂量的GE或一定剂量的维生素E并一定量的COF ,观察淋巴细胞的姐妹染色单体交换率(sisterchromatidexchanges,SCE)的变化 ,挑选GE(按1:10000稀释)和维生素E(按1:1000稀释)同时注入培养基中(含COF10μl) ,观察二者对COF的协同抗突变作用。 结果 :GE(按1:10000稀释)可显著降低COF处理过的淋巴细胞SCE(P<0.05) ;维生素E(按1:1000稀释)和GE(按1:10000稀释)在降低COF处理过的淋巴细胞SCE方面存在着协同作用(P<0.01)。 结论 :GE和维生素E在拮抗COF的致突变性过程中存在着显著的协同效应 ;一定量的GE亦有一定的拮抗COF的致突变作用。更多还原

【Abstract】 Purpose: To observe antagonistic effects of gar li c extract(GE)and vitamin E to mutagenicity of condensates of coo king oil fumes(COF). Methods: SCE(sister chromatid exchange) wa s observed and compared before and after addition of garlic e xtract or vitamin E in different amounts to the culture of human peripheral blood lymphocytes containing different amou nts of COF. Then GE(1:10 000 dilution)and / or vitamin E (5 μl) were added to the culture of human peripheral blood lympho cytes containing COF 10μl to compare the changes in SCE. Results: Garlic extract(1:10 000 dilution) could lower SCE of lymphocytes(P< 0.05); Garlic extract and vitamin E had a very si gnificantly synergistic effect on lowering SCE of lymphocytes t reated by COF(P< 0.01). Conclusions: Garlic extract and vitamin E have a synergistic effect to antagonize the mutagenicity of COF; garlic extract alone can also lower SCE of h uman peripheral blood lymphocytes.更多还原