【摘要】 目的 :探讨三氯乙烯(TCE)对职业人群有无细胞遗传损伤作用以及与接触浓度和时间的关系。 方法 :采用气相色谱和吡啶化学比色法对工作场所中TCE浓度和工人尿中三氯乙酸(TCA)含量进行了测定 ,采用外周血淋巴细胞微核试验、胞质分裂阻滞微核试验以及姐妹染色单体交换(SCE)试验、单细胞凝胶电泳(SCGE)试验对141名直接接触TCE的工人和39名对照者检测了有无染色体损伤和DNA损伤。 结果 :TCE接触组人群(车间内空气中TCE平均浓度为90.4mg/m3 ,尿中TCA平均浓度为61.79mg/L)微核、双核微核、SCE和彗星样淋巴细胞出现率分别为1.66‰、2.73‰、4.33、7.48 % ,均明显高于对照组(分别为1.13‰、1.66‰、2.95、3.74 % ,P<0.05或P<0.01) ;除SCE外 ,微核、双核微核、彗星样淋巴细胞出现率与接触工龄间存在相关关系(r=0.222 ,0.246 ,0.320 ;P<0.05或P<0.01) ;微核、双核微核发生率、SCE和彗星样淋巴细胞出现率与尿中TCA浓度间也存在相关关系(r=0.294 ,0.260 ,0.229 ,0.268 ;P<0.05或P<0.01)。 结论 :TCE具有遗传毒作用 ,长期接触高浓度TCE可导致染色体断裂和DNA损伤更多还原

【Abstract】 Purpose: To evaluate the cytogenetic effects of trichloroethylene(TCE) on the workers, and to determine the dose_response and time_response correlations among the exposure level and duration. Methods:TCE concentration in the work place and TCA in urine of the workers exposed to TCE were determined using gas chromatography and pyridine spectrophotometry, respectively. Conventional micronucleus(MN),cytokinesis_block micronucleus(CB_MN), sister_chromatid exchanges(SCE), and single cell gel electrophoresis (SCGE) from 141 workers exposed to TCE and 39 controls were carried out to determine chromosomal and DNA damage. Results: In the TCE_exposed group(the average concentration of TCE in work place was 90.4 mg / m 3, the average level of urine TCA of the workers exposed to TCE was 61.79 mg / L), the frequencies of MN, CB_MN, SCE and the percentage of lymphocytes with comet_like tail in peripheral blood lymphocytes(PBLs) were 1.66 ‰, 2.73 ‰, 4.33 and 7.48 %, respectively, and were higher than those in the control groups(1.13 ‰, 1.66 ‰, 2.95 and 3.74 %, respectively, P<0.05 or P<0.01). Except for SCE, there were positive correlations between the percentage of MN,CB_MN, the percentage of lymphocytes with comet_like tail in PBLs and the exposing time(r=0.222, 0.246, 0.320; P<0.05 or P<0.01). The positive correlations were also evidant between the percentage of MN,CB_MN, SCE and the percentage of lymphocytes with comet_like tail in PBLs and the the average level of urine TCA of the workers exposed to TCE (r=0.294, 0.260, 0.229, 0.268; P<0.05 or P<0.01). Conclusions: TCE is genotoxic, and long term exposure to the high dose of TCE may induce chromosome breakage and DAN damage.[更多还原