【摘要】 层状材料—α-磷酸锆(α-ZrP)固定化青霉素酰化酶(PGA)开辟了固定化载体研究的新领域。以α-ZrP作载体,用直接吸附法、客体插层和戊二醛交联等探索出一条适合于此载体的固定化方法,并对此固定化酶进行了研究。制成的固定化酶的表观活力达521 U/g,连续水解20批青霉素钾盐溶液,其活力未见有明显下降,酶活回收率达37.4%。更多还原

【Abstract】 It was opening a new field of immobile carrier to use layer material——α-Zirconium phosphate on the immobilization of penicillin G acylase. This issue described a method of immobilization, use α-ZrP as carrier, with direct absorption, guest carrier intercalation, glutaraldehyde crosslink, etc, and study on the immobilized acylase. The activity of the immobilized penicillin acylase was about 521 U/g, whose activity had no obvious decrease after 20 continuous hydrolization of penicillin G. The yield of the immobilization of penicillin G acylase was 37.4%.更多还原