【摘要】 目的 :观察中药复方复聪片及拆方后方内补肾、益气养血、活血祛瘀、通窍聪耳等主要药物对豚鼠庆大霉素 (GM )耳毒性反应的拮抗作用。方法 :豚鼠每日肌肉注射GM80mg/kg,同时每日喂服复聪片或等效剂量各拆方组药物 ,连续 2 0日 ,测定用药前后听觉脑干诱发电位 (ABR)阈值 ,耳蜗基底膜铺片外毛细胞计数 ,螺旋韧带铺片观察血管纹血流状况及耳蜗超微结构电镜观察等 ,评价药物的拮抗效果及对耳蜗结构的保护作用。结果 :复聪片及其拆方之补肾、益气养血、活血祛瘀药物可减轻GM所致耳蜗外毛细胞损伤率 (P <0 0 5 ) ,保护耳蜗结构及功能 ,拮抗GM所致之听阈升高 (P <0 0 5 )。结论 :复聪片及方中补肾、益气养血、活血祛瘀药物可有效减轻GM的耳毒性反应。更多还原

【Abstract】 Objective: To assess the antagonistic effect of Fucong Tablet (FCT) and its ingredients on ototoxicity of gentamycin in guinea pigs. Methods: The guinea pigs were given gentamycin 80mg/kg intramuscularly once daily for 20 days and FCT or its ingredients separately oral taken for 20 days. The auditory brain stem evoked response (ABR) threshold, vascular stria blood circulation condition, outer hair cell count and cochlear ultrastructure of guinea pigs were observed before and after treatment to assess the antagonistic effect and protective effect of FCT on conchlear structure. Results: FCT and its ingredients of tonifying Kidney, replenishing Qi and blood,promoting blood circulation could lower the impairment ratio of outer hair cell induced by gentamycin (P<0 05), protect morphosis and function of cochlea and lower the elevated ABR threshold (P<0 05). Conclusions: FCT and its ingredients could reduce the toxic damage on ear induced by gentamycin effectively.更多还原