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一阶导数紫外分光光度法测定茶碱的血药浓度

Determination of Theophylline in Human Serum by First Order Derivative UV-Spectrophotometry Method

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【作者】 肖桂珍罗顺德张先洲施美君

【Author】 Xiao Guizhen, Luo Shunde, Zhang Xianzhou, et al Zhuhai People’s Hospital, Zhuhai 519000, Guangdong, P.R.China

【机构】 广东省珠海市人民医院湖北医科大学药学系湖北医科大学药学系 519000

【摘要】 采用一阶导数紫外分光光度法进行茶碱血药浓度测定,此法灵敏度高,专一性强,线性良好(r=0.9999,回收率较高,变异性小(RSD<5%)。用于临床茶碱血药浓度监测,方法简便准确,并收到满意效果。

【Abstract】 The theophylline concentration by a first order derivative UV-spectrophotometry in human serum was determined. The method had advantage of higher sensitivity, stronger specificity, and better linearity (r=0.999 9). The rate of recovery was higher (total CV<5%), the variability was less. This is a better examining method for clinical theophylline serum concentration monitoring, and being simple and presise.

  • 【分类号】R96
  • 【被引频次】2
  • 【下载频次】82
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