【摘要】 目的：研究神经生长因子（ＮＧＦ）对缺血性脑损伤中神经细胞凋亡的影响。方法：采用大鼠全脑缺血模型，ＴＵＮＥＬ方法原位标记ＤＮＡ片段，观察脑室内注射ＮＧＦ后海马ＣＡ１区神经细胞凋亡的变化。结果：使用ＮＧＦ后３日海马ＣＡ１区ＴＵＮＥＬ阳性神经细胞数为锥体细胞总数的１３．９％±１１．６％，与缺血对照组（２１．３％±１３．７％）比较显著减少（Ｐ＜０．０１）。结论：外源性ＮＧＦ对脑缺血所致的神经细胞凋亡可能具有一定的保护作用更多还原

【Abstract】 Objective:To investigate the protective effect of nerve growth factor(NGF) on neuronal apoptosis induced by ischemic insults.Methods:Global ischemia in Wistar rats was induced using fourvessel occlusion,and animals were divided into NGFtreated group (intraventricular administration of NGF) and control group (same volume of vehicle).On day 3 after ischemia,neuronal apoptosis was determined by terminal deoxynucleotidyltransferase mediated dUTPbiotin nick end labeling (TUNEL) in hippocampal CA1 sector.Results:The number of TUNEL positive cells to the number of whole cells in the CA1 sector was 213%±137% in the control group and 139%±116% in NGFtreated group respectively,and the difference was significant(P<001).Conclusions:It is suggested that intraventricular administration on NGF has a protective effect on neuronal apoptosis associated with cerebral ischemic insults.更多还原