【摘要】 菠菜（ＳｐｉｎａｃｉａｏｌｅｒａｃｅａＭｉｌ．）品种“华菠一号”生长在室内条件下，以０．０和１３．０ｋＪ·ｍ－２·ｄ－１的ＵＶＢ进行照射处理，研究了ＵＶＢ对菠菜叶片内类黄酮和脂质过氧化作用的影响。结果表明：ＵＶＢ辐射降低了菠菜的单株叶鲜重，诱导了体内吸收ＵＶＢ的类黄酮化合物的大量积累，并使可溶性蛋白含量与叶绿素含量下降。ＵＶＢ辐射能使菠菜叶片内Ｏ－·２及丙二醛（ＭＤＡ）含量大幅度上升，而使抗坏血酸（ＡｓＡ）含量下降。ＵＶＢ辐射能使菠菜叶片内过氧化物酶（ＰＯＸ）、ＳＯＤ的活性大幅度上升，而过氧化氢酶（ＣＡＴ）活性仅略有增加。初步推测：ＵＶＢ辐射首先诱导菠菜叶片内Ｏ－·２的积累，并导致膜脂质过氧化作用，从而抑制菠菜生长。Ｏ－·２的积累能诱导抗氧化酶活性的上升，但抗氧化酶活性的上升和吸收ＵＶ的类黄酮化合物的积累并不能扭转ＵＶＢ对菠菜的伤害。更多还原

【Abstract】 Spinach (Spinacia oleracea Mill.) cultivar “Huabo No.1” was grown in an indoor environment and treated with 13.0 kJ·m-1·d-1 of ultravioletB (UVB 280 to 320 nm) to study the effect of UVB irradiation on flavonoids and lipid peroxidation in spinach leaves. The results showed that enhanced UVB irradiation decreased the leaf fresh weight and the content of soluble protein and chlorophyll, and induced large accumulation of UVabsorbing flavonoids in the leaves. UVB irradiation also promoted the production of superoxide radicals (O-·2) and malondialdehyde in spinach leaves. However, the ascorbic acid (AsA) level was decreased under UVB treatment. It was interesting that high peroxidase (POX), superoxide dismutase (SOD) and catalase (CAT) activities in spinach leaves were induced by UVB irradiation, the former two being more sensitive. It was suggested that UVB induced the accumulation of O-·2 resulting in the lipid peroxidation and in turn inhibiting the growth of spinach. However, the increase of UVabsorbing flavonoids and antioxidative enzymes induced by high accumulation of O-·2 could not reverse the process of UVB damage.更多还原