Using PCR method the rolC gene was amplified from Agrobacterium rhizogenes,and CaMV 35S/rolC expression vector pCaR was constructed. The chimeric gene via agrobacterium mediated procedure was transformed separately into the wild type tobacco (Nicotiana tabacum L. cv. W38) and the transgenic tobacco of ipt gene. The putative transgenic plants were assayed with Southern blot and RNA Dot blot analysis. The observation suggested that the transgenic tobacco exhibited the abnormal phenotypes as a consequence of ...
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