【摘要】 有研究表明ＡＣＥ基因（分ＤＤ、ＩＤ、ＩＩ三种基因型）与心血管疾病（如高血压、左心室肥大、心肌梗塞等）关系密切。为探讨ＡＣＥ基因多态性与高血脂的可能关系，在上海华山医院老干部体检中心体检者中抽取３１名高胆固醇患者和３４名血脂正常者作对照研究，利用对象的血凝块提取ＤＮＡ，经ＰＣＲ扩增和琼脂糖凝胶电泳检测其ＡＣＥ基因型。实验结果显示，患者组与对照组间ＡＣＥ基因型分布无显著性差异，等位基因频率也无显著性差异，结果提示ＡＣＥ基因与高血脂可能没有联系，等位基因Ｄ可能不是高血脂的一个潜在危险因素，但与样本量较小有关，有待于进一步研究。更多还原

【Abstract】 It has been reported that the isertion (I)/deletion(D)Polymorphism of the angiotensinconverting enzyme(ACE)gene is strongly associated with coronary heart disease(CHD)(such as hypertension left ventricular hypertrophy myocardial infarction).The objective of this study is to assess the rletionship between ACE gene I/D polymorphism and hyperlipidemia.We selected 31 subjects with hyperlipidemia and 34 control subjects without hyperlipidemia from the older people who enrolled yearly health examination in Huashan hospital.The genomic DNA was isolated from blood blot and the ACE genotype was analyzed by polymerase chain reaction.Plsama total cholesterol was measured by enzymatic method.Results:No difference was observed between subjects with hyperlipidemia and controls in ACE genotype frequency(χ2=134 P>0050),Nor in the frequency of allele(χ2=124 P>005 OR=153,95%CI 068～346)。Conclution:These findings suggested that ACE gene polymorphism is not associated with the total cholesterol levels in both subjects with hyperlipidemia and controls.The allele D may not be the risk factor of hyperlipidemia.更多还原