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灰葡萄孢毒素的组分分析和生物测定
ANALYSIS OF TOXICITY COMPOSITION AND BIOASSAY OF BC TOXIN FROM BOTRYTIS CINEREA
【摘要】 用PD培养液培养灰葡萄孢菌,过滤液中含活性成分,经氯仿提取后TLC分离获6种组分,Rf值分别为Ⅰ(0.36)、Ⅱ(0.48)、Ⅲ(0.54)、Ⅳ(0.65)、Ⅴ(0.11)、Ⅵ(0.77),经针刺果实生物测定发现,Ⅲ、Ⅴ、Ⅵ具有毒性。BC-毒素具有一定的广谱性,对不同科属的番茄、茄子、黄瓜、白菜、油菜、小麦、玉米、棉花等都具有不同程度的毒害作用。
【Abstract】 Botrytis cinerea was cultured on PD medium for 21 days, and culturefiltrates contained activity components. The crude BC toxin form chloroformextraction was separated into six separates by TL chromatography,ie. Ⅰ( Rf0.36) Ⅱ(Rf0.48) Ⅲ(Rf0.54) Ⅳ(Rf0.65) Ⅴ(Rf0.11)Ⅵ(Rf0.77),among them, Ⅲ、Ⅴ、Ⅵ had toxicity by fruit bioassay.The experimental results also showed that BC toxin had toxicity to many plants, such as,tomato, eggplant,cucumber,chinese cabbage, rape,corn,wheat,cotton and so on.
- 【文献出处】 植物病理学报 ,ACTA PHYTOPATHOLOGICA SINICA , 编辑部邮箱 ,1998年03期
- 【分类号】S432.42
- 【被引频次】102
- 【下载频次】311