【摘要】 目的：探讨骨巨细胞瘤（ＧＣＴ）各细胞成分及肿瘤坏死因子α（ＴＮＦα）和白细胞介素１（ＩＬ１）细胞因子在骨吸收中的作用。方法：采用细胞分离技术和体外细胞溶骨模型，检测ＧＣＴ各细胞成分的骨吸收活性及ＴＮＦα和ＩＬ１对这种活性的影响。结果：在溶骨模型中，多核巨细胞（ＭＧＣ）的数目越多，４５Ｃａ２＋的释放也越多；纤维母细胞样基质细胞（ＦＣ）组的４５Ｃａ２＋值明显高于无细胞对照组（Ｐ＜０．０１），而与骨肉瘤细胞组比较，无显著差异；此外，ＦＣ的条件培养液能大大增强ＭＧＣ溶骨模型中４５Ｃａ２＋的释放（Ｐ＜０．０１）；原代单核基质细胞（ＳＣ）经ＴＮＦα处理后，其模型中４５Ｃａ２＋释放明显增高（Ｐ＜０．０１），而ＩＬ１未见类似作用。结论：在ＧＣＴ中，多核巨细胞具有直接溶骨的作用；纤维母细胞样基质细胞，除直接溶骨外，还可以分泌某种物质促进多核巨细胞的溶骨效应；ＴＮＦα能增强原代单核基质细胞的溶骨能力。更多还原

【Abstract】 Objective: To explore the bone resorption activity of different cell components in Giant cell tumor of bone （GCT） with reference to the effect of Tumor necrosis factor-α（TNF-α） and Interleukin-1（IL-1）. Methods:Cell separation technics and in vitro model of bone resorption by cells were used to detect the osteolytic capability of different cell components in GCT. The function of TNF-α and IL-1 in GCT’S bone resorption were also detected. Results: In bone resorption model, the more multinucleated giant cells（MGC） present, the higher will be the level of⁴⁵Ca²+ released. The ⁴⁵Ca²+ levels of fibroblastlike stroma cell（FC） group were significantly higher than that of no cell’s group （P<0.01）, while there was no significant difference between FC group and osteosarcoma cell group. In addition, the conditioned medium of FC could enormously increase the levels of ⁴⁵Ca²+ released from MGC’s model （P<0.01）. After treatment by TNFα, the stroma cells could increase the levels of ⁴⁵Ca²+ released from it’s bone resorption model （P<0.01）, but IL-1 did not have similar effect. Conclusions:In GCT, the multinucleated giant cells has the ability to resorb bone matrix directly. The fibroblastlike stroma cells could not only resorb bone matrix directly, but also secret unknow material to promote bone resorption of multinucleated giant cells. TNF-α has the ability to promote bone resorption of GCT’s stroma cells.更多还原