【摘要】 为研究高脂高胆固醇膳食对动脉壁内皮一氧化氮（ＮＯ）合成的影响，以高脂高胆固醇饲料喂饲雄性纯种新西兰兔８周后，通过定量逆转录多聚酶链式反应（ＲＴ－ＰＣＲ），观察高脂高胆固醇膳食对动脉组织一氧化氮合成酶（ＮＯＳ）基因表达影响。结果表明：高脂高胆固醇膳食８周后，内皮型（ＮＯＳ）ｍＲＮＡ表达下降、总ＮＯＳ活力减弱、血浆一氧化氮代谢产物（ＮＯＰ）水平降低（Ｐ＜０．０５）；内皮素（ＥＴ）水平上升。结果提示：高脂高胆固醇膳食引起高脂血症，可损害动脉内皮，降低ＮＯＳ的基因表达，抑制ＮＯ的合成更多还原

【Abstract】 To study the effects of high dietary fat and cholesterol on NO synthesis of artery endothelium, 20 male pure New Zealand rabbits were assigned randomly to receive normal diet or high fat and cholesterol diet for 8weeks. Results: severe damage of aortic endothelial cells and atherosclerotic lesions were observed in the study group. Conpared with the control group, the activity of endothelial NO synthetase, its mRNA expression and serum level of NO products decreased, while plasma endothelin in creased in study group. It indicated that high dietary fat and cholesterol could impair artery endothelium and inhibit its nitric oxide synthesis in rabbits. Author’s address Li Yong, Shanghai Medical University, Shanghai 200040 PRC.更多还原