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HPLC反相色谱法快速测定冬虫夏草中的腺苷

HPLC reversed phase chromatography for rapid determination of adenosine in Cordyceps Sinensis.

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【作者】 赵珊; 罗仁才; 涂晓明;

【Author】 Zhao Shan Luo Rencai Tu Xiaoming

【机构】 北京市卫生防疫站;

【摘要】 为建立保健食品中腺苷的测定方法,对冬虫夏草为原料的保健食品中的腺苷进行超声提取,采用反相HPLC法,磷酸二氢钾缓冲液加甲醇(90+10)做流动相,254nm波长下检测腺苷,分离效果好且快速,回收率及精密度均较高,适于推广。

【Abstract】 rapid method for determination of adenosine in Cordyceps Snensis in health food was developed . The adenosin in the samples was extracted by means of ultrasonic wave and detected by HPLC reversed phase chromatography, potassium dihydrogen phosphate buffer solution:methyl alcohol(90:10) as mobile phase and detection at 254nm. This method has high selectivety and is suitable for rapid and accurate analysis of adenosine in health foods. Author’s address Zhao Shan, Health and Anti-epidemic Station of Beijing, Beijing 100013, PRC.

  • 【文献出处】 中国食品卫生杂志 ,CHINESE JOURNAL OF FOOD HYGIENE , 编辑部邮箱 ,1998年01期
  • 【分类号】R927.2
  • 【被引频次】6
  • 【下载频次】136
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