【摘要】 目的：通过构建人扁桃体淋巴细胞cDNA质粒重组体，为深入研究淋巴细胞的生物学功能奠定实验基础。方法：采用DNA重组技术，以人新鲜扁桃体淋巴细胞为实验材料从中提取mRNA，以mRNA为模板合成cDNA，然后与质粒pSPORTI进行定向连接并转入到E．coliJM109中进行分子克隆。结果：阳性克隆占90％，阴性克隆占10％；转化率为1．35×104克隆／微开连接体系；插入片段长度为0．5～2．3Kb，平均1．3Kb。结论：人扁桃体淋巴细胞cDNA质粒重组体构建成功。更多还原

【Abstract】 Objective: To lay a foundation for the test of biochemistry mechanism of the lymph9cytes, we constructed of plasmid recombinant of the human tomsil lymphocytes cDNA. Method: DNA recombinant technique was used. The lymphocytes of the human fresh tonsils were used as the materials for the test and mRNA was purified from the materials, then mRNA being the template, cDNA was synthesized. The fragments of cDNA were inserted into the plasmid pSPORT I in only one orientation to form recombinant DNA molecules, which were then introduced to E. coli JM 109. Result:90 percent were positive clones and 10 percent were negative therefore, the transformation efficiency of each microliter ligation reactions was 1.35 × 104 clones. The length of fragments inserted was from 0. 5 to 2.3Kb, average value 1.3Kb. Conclusion: Plasmid recombinant of the human tonsil lymphocytes cDNA has been successfully established.更多还原