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紫外分光光度法测定头孢他啶脂质体的包封率与渗漏率

Determination of encapsulation efficiency and release rate of ceftazidime liposome by UV spectrophotometry

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【作者】 罗云敬沈含熙杜鸣孔德明

【Author】 Luo Yunjing, Shen Hanxi, Du Ming and Kong Deming (Department of Chemistry, Nankai University, Tianjin 300071)

【机构】 南开大学化学系

【摘要】 结合葡聚糖凝胶微型柱和平衡透析两种分离方法,采用紫外分光光度法测定了头孢他啶脂质体的包封率与渗漏率。在0.18~6mg/L,头孢他啶浓度与吸光度呈良好线性关系,r=0.9999。加标回收率大于99%,RSD小于0.5%(n=5)。

【Abstract】 An UV spectrophotometric method was established for the determination of encapsulation efficiency and release rate of ceftazidime, combining with separated methods of dialysis and a minicolumn of Sephadex G 50. Linear relationship was found in the ceftazidime concentration ranging from 0.18 to 6 mg/L. The average recovery was more than 99%, and the relative standard deviation was less than 0.5%. The method is practicable.

【基金】 国家自然科学基金
  • 【文献出处】 中国抗生素杂志 ,CHINESE JOURNAL OF ANTIBIOTICS , 编辑部邮箱 ,1998年03期
  • 【分类号】R927.1
  • 【被引频次】14
  • 【下载频次】277
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