【摘要】 目的：揭示ＩＦＮ－γ抗弓形虫作用的剂量相关性及ＴＮＦ－α对ＩＦＮ－γ是否存在协同作用。方法：本实验体外以不同剂量ＩＦＮ－γ单独或与ＴＮＦ－α联合刺激昆明鼠腹腔巨噬细胞，观察其对入侵的ＲＨ株速殖子的作用及培养上清中ＮＯ的水平。结果：随着ＩＦＮ－γ剂量的增大，巨噬细胞抗虫作用增强，培养上清中ＮＯ水平增高；虫体入侵后２４ｈＮＯ水平与细胞内虫体数呈显著负相关。结论：ＩＦＮ－γ可激活巨噬细胞抑制和杀灭入侵的弓形虫，并与剂量有关，ＴＮＦ－α对此有协同作用，ＮＯ是抗虫作用中重要的效应因子更多还原

【Abstract】 AIM: To find out the dose dependence of the anti Toxoplasma effect induced by IFN γ and to determine the possible synergistic activity between TNF α and IFN γ. METHODS: The in vitro effect of cultivated mouse peritoneal macrophages activated by IFN γ alone or IFN γ combined with different doses of TNF α on the intracellular tachyzoites of RH strains and the nitric oxide (NO) level in the culture medium supernatant were simultaneously determined. RESULTS:With the increase in the dose of IFN γ,the anti Toxoplasma effect was augmented and the NO level was enhanced. At 24 hours after tachyzoite invasion, a significant reversed correlation was demonstrated between the NO level and the number of intracellular parasites. CONCLUSION: The anti Toxoplasma effect of macrophages activated by IFN γ appears to be dose dependent and TNF α acts synergitically with IFN γ in the activation of macrophages. The production of reactive NO could be an important effector in the IFN γ primed anti Toxoplasma action.更多还原