【摘要】 自稻叶及其它禾本科杂草叶片表面分离纯化９８５株细菌分离物，分别培养在ＮＢ液体培养基中，经８０００ｒ／ｍｉｎ离心１０ｍｉｎ后取上清液，加入稻瘟病菌分生孢子悬液中，测定对稻瘟病菌分生孢子发芽，附着胞形成的抑制作用，从而筛选出１３株对稻瘟病菌孢子芽管生长或附着胞形成具有抑制作用的拮抗细菌．采用离体稻叶接种测定，进一步筛选得到３株拮抗细菌，初步鉴定为：Ｐｓｅｕｄｏｍｏｎａｓｆｌｕｏｒｅｓｃｅｎｓ（Ｘｈ２１６），Ｂａｃｉｌｕｓｓｐ．（Ｘｈ２２２）及Ｐｓｅｕｄｏｍｏｎａｓｓｐ．（Ｘｈ２４０）．在离体条件下，Ｘｈ２１６，Ｘｈ２２２和Ｘｈ２４０对叶瘟的防治效果分别为７２．３％，７０．５％和６７．４％．更多还原

【Abstract】 A total of 985 bacterial isolates were obtained from rice leaves and other grasses. The isolates were cultared in nutrient broth(NB) medium. After centrifugated, 8 000 r/min for 10 min, the supernatants of each bacterial NB cultures were tested for their suppressive effects on the germination and appressorium formation of Magnaporthe grisea . Of the tested isolates, 13 isolates showed suppressive effects. Three isolates identified as Psendomonas fluorescens (Xh216), Bacillus sp. (Xh222) and Pseudomonas . sp. (Xh240),suppressed the disease severities on the detached rice leaves with the disease severity reduction 72.3%, 70.5% and 67.4%, respectively.更多还原