【摘要】 以水稻抗瘟性近等基因系Ｈ７Ｒ和Ｈ７Ｓ为材料，采用ＰＣＲ－差别筛选（ＰＣＲｂａｓｅｄｄｉｆｅｒ－ｅｎｔｉａｌｓｃｒｅｅｎｉｎｇ）的方法分离和克隆水稻受稻瘟病菌诱导的抗性相关基因．第一轮筛选获得４２个差异表达的ｃＤＮＡ克隆，经ＰＣＲ扩增，转膜，反向Ｎｏｒｔｈｅｒｎ杂交后，得到１２个阳性ｃＤＮＡ克隆．本文对ＰＣＲ－差别筛选的优缺点以及进一步的鉴定工作亦作了讨论．更多还原

【Abstract】 A PCR based differential screening method was employed to isolate and clone blast resistance related genes induced by Magnaporthe grisea with a pair of rice near isogenic lines (NIL) H7R and H7S as materials . From the primary differential screening step , a total of 42 impure pools of candidates were selected. Each pool of clones was amplified by PCR and then the PCR products were blotted onto filters. Twelve positive cDNA clones were obtained after reverse Northern hybridization. The advantages and disadvantages of PCR based differential screening method and further characterization were also discussed.更多还原