【摘要】 本文对大陆株日本血吸虫２６ｋＤａ谷胱甘肽Ｓ－转移酶（Ｓｊ２６ＧＳＴ）的ＤＮＡ疫苗进行研究，并观察了其免疫保护效果。分别构建含Ｓｊ２６ＧＳＴ基因的重组质粒ｐＣＤ－Ｓｊ２６和ｐＢＫ－Ｓｊ２６，采用脂质体介导的基因转移将ｐＣＤ－Ｓｊ２６和ｐＢＫ－Ｓｊ２６分别导入ＮＩＨ３Ｔ３成纤维细胞，用免疫荧光法（ＩＦＡ）证实Ｓｊ２６ＧＳＴ在真核细胞中的表达。分别将ｐＣＤ－Ｓｊ２６和ｐＢＫ－Ｓｊ２６及ｐＢＫ－ＣＭＶ肌注ＢＡＬＢ／ｃ小鼠。免疫３次后，免疫鼠产生一定水平的抗血吸虫抗体。用日本血吸虫尾蚴攻击感染免疫小鼠，结果ｐＣＤ－Ｓｊ２６和ｐＢＫ－Ｓｊ２６免疫鼠减虫率分别为３７．１９％和４４．４４％，肝组织减卵率为７３．８０％和４７．２０％，每对成虫产卵减少５６．８８％和１５．６７％。实验结果表明，日本血吸虫Ｓｊ２６ＤＮＡ疫苗免疫小鼠后能诱生一定水平的抗血吸虫抗体，免疫鼠可形成一定水平的保护性免疫力，预防血吸虫尾蚴感染。同时可减轻宿主肝组织血吸虫卵所致的病理损害作用更多还原

【Abstract】 DNA vaccines, the antigenencoding DNAs used in vaccination, represent a novel approach to the development of subunit vaccines. In this study,the plasmid pCDSj26 and pBKSj26,both of which contain the fulllength cDNA of 26 kDa glutathione Stransferase of S. japonicum(Sj26GST)were constructed. The pCDSj26 and pBKSj26 were transiently transfected into NIH3T3 cells. The expression of Sj26GST in transfected cells was confirmed by indirect immunofluoresent assay. The BALB/c mice were inoculated by intramuscular injection with pCDSj26,pBKSj26 and pBKCMV(control plasmid), respectively. Mice immunized with DNA vaccine developed a certain level neutralizing antibody to S. japonicum. Vaccination with pCDSj26 and pBKSj26 resulted in significant protection with 37.19% and 44.44% reduction in worm burden,73.80% and 42.20% reduction in liver tissue eggs, 56.88% and 15.67% reduction in female worm fecundity, respectively. The results indicate that Sj26 GST DNA vaccine can induce a certain level of specific protective immunity in mice against infection with S. japonicum and an antifecundity, theredy reducing pathology.更多还原