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作物种子纯度检验的电泳方法

Electrophoresis Methods of Testing Seed Purity in Field Crops

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【作者】 吴正三陈明

【Author】 Wu Zhengshan; Chen Ming (Anhui Agricultural University, Hefei 230036 ) (Seed Company of Shouxian County, shouxian county232200)

【机构】 安徽农业大学!合肥230036寿县种子公司!寿县232200

【摘要】 分析比较了应用于作物种子纯度检验的几种电泳方法的特点,过程及限制因素。结果表明:同工酶电泳法速度快、成本低,而提供的多态性有限;胚乳或种胚蛋白质电泳法简单易行、在一些作物上具丰富的多态性;DNA电泳法(分子标记)能提供最丰富的多态性,但目前分析过程还较复杂.费用也较昂贵。

【Abstract】 The characteristics, procedures and limitations of several electrophoresis methods for testing seed purity in field crops were presented in this paper. The results showed that isozyme PAGE was rapid and inexpansive, but the munber of isozyme was lirnited. Endersperm of embryo protein PAGE was simple and a great deal of protein band could be found. DNA molecular markers had frequent variations, but the screening procedures were complex and costly.

【关键词】 作物种子纯度检验电泳
【Key words】 Fidld crop seed purity test electrophersis
  • 【分类号】S339.3
  • 【被引频次】14
  • 【下载频次】96
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