【摘要】 目的探讨黄芩甙对大鼠脑匀浆铁依赖脂质过氧化（ＩＤＬＰＯ）的抑制作用和对百日咳致大鼠脑水肿的保护作用。方法用ＳＤ大鼠脑匀浆观察不同浓度黄芩甙对ＩＤＬＰＯ的抑制作用。体内实验采用百日咳菌液注入颈内动脉导致大鼠脑水肿，观察黄芩甙的保护作用。动物随机分为生理盐水组（ＮＳ）、百日咳菌液组（ＰＢ）、去铁胺组（ＤＦＸ）和黄芩甙（ＢＡＩ）治疗４组。结果脑匀浆实验显示黄芩甙对ＩＤＬＰＯ的抑制作用较ＤＦＸ约强２０倍。体内实验ＤＦＸ和ＢＡＩ２组注射百日咳菌液侧（左）大脑半球的含水量（７９８％±０７）％和（７９５±０６）％、伊文思蓝含量（１１±７）μｇ／ｇ和（１２±５）μｇ／ｇ）和铁含量（７９±９μｇ·ｄ－１·ｇ和７７±１０μｇ／ｄｒｙｇ）均较ＰＢ组同侧大脑半球（８０４±０９）％、２４±１０μｇ／ｇ和１３２±２８μｇ·ｄ－１·ｇ）为低，Ｐ＜００５。ＰＢ组２侧脑组织ＣｕＺｎＳＯＤ活性（左１５５±３６Ｕ／ｍｇ，右１７７±７５／ｍｇ）均较ＮＳ组（左２０１±４９Ｕ／ｍｇ，右２４９±４９Ｕ／ｍｇ）为低（Ｐ＜００５），在ＤＦＸ组和ＢＡＩ组左侧脑组织ＣｕＺｎＳＯＤ活性（分别为２２２±４９Ｕ／ｍｇ和２３０±４５Ｕ／ｍｇ）均较Ｐ?更多还原

【Abstract】 Objective To evaluate the inhibitory effect of baicalin on irondependent lipid peroxidation (IDLPO) and the protective effect on pertussis bacilliinduced brain edema in rats. Methods In vitro, the inhibitory effect of baicalin and deferoxamine (DFX) was studied at different concentrations on IDLPO in brain homogenates of SpragueDawley rats. In vivo, the protective effect was studied of baicalin and DFX on brain edema induced by injecting purtussis bacilli into the left internal carotid artery in rats. Forty SpragueDawley rats were randomly divided into four groups. Group 1 (NS, n=10), as control; Group 2 (PB, n=10), pertussis bacilli control; Group 3 (DFX, n=10), PB + deferoxamine; and Group 4 (BAI, n=10), PB + baicalin. Results The baicalin was possessed of an inhibitory effect on IDLPO in rat brain homogenate, moreover, the inhibitory potency of baicalin was approximately 20 times higher than DFX treated alone. In vivo, the increase in the content of water(CW) and Evans blue (EB) was attenuated in groups DFX and BAI (CW: 79.8±0.7) % and (79.5±06)%; (EB:11±7μg/g and 12±5 μg/ g ) as compared with the group PB (CW: 80.4±0.9 %, EB:24±10 μg/g). The content of iron was significantly lower than in the left hemispheres (injected side) in the group DFX and the group BAI (79±9 μg/ g and 77±10 μg/ g ) than in the left hemispheres in the group PB(132±28 μg·d-1·g ), P< 0.05。The activation of CuZnSOD (L155±36 U/mg pr,R177±75 U/mg pr) was decreased in the group BP compared with the group NS(L 201±49 U/mg pr, R 249±49 U/mg pr ), P<0.05. The activation of CuZnSOD was significantly higher in the left brain tissues in the groups DFX and BAI (222±49 U/mg pr and 230±45 U/mg pr, respectively) than in the left brain tissues of the group of PB (P<005). Conclusion The baicalin and DFX have a protective effects on the pertussis bacilliinduced brain edema in rats, and the protective effects are related to the inhibitory effect on IDLPO, chelating Fe2+, and activated CuZnSOD.更多还原