【摘要】 目的研究血清铜络合蛋白（ＣＣＰ）在癌症患者的改变。方法人血清经Ｃｕ２＋处理后，通过１４％聚丙烯酰胺凝胶（ＰＡＧＥ，交联度１％）垂直平板电泳技术检测ＣＣＰ蛋白质区带的变化。结果经Ｃｕ２＋处理后的血清较原血清电泳区带新增一条区带，即ＣＣＰ带；发现在３３８例癌症患者电泳区带中ＣＣＰ（％）为２３±０８，显著低于１４６例正常人（５８±１４）和９５例非癌症患者（５４±１６，Ｐ＜００１），尤其在恶性淋巴瘤患者减低更为显著，与正常组和非癌症患者组差异无显著意义。实验发现ＣＣＰ的产生可能与富含巯基的白蛋白有关。结论ＣＣＰ可能在肿瘤的辅助诊断和疗效观察中有一定价值更多还原

【Abstract】 Objective To study the changes of copper complex protein (CCP) in cancer patients.Methods After human serum was treated with ionic copper, protein zones were separated by 14% PAGE (crosslinkage rate 1%) vertical slab electrophoresis.Results A new zone was detected in the treated serum with ionic copper. In the CCP zone of 338 cancer patients, 146 normal controls, and 95 noncancer patients the percentage of CCP was 2.3±0.8, 5.8±14, 5.4±1.6, respectively. Cancer patients showed significantly reduced CCP values (P<0.01), especially in those with malignant lymphoma. There was no significant difference between the percentage of CCP in normal controls and noncancer patients. The origin of CCP was possibly related to albumin binding mercaptoethanesulphonic acid (mesna) in experiment.Conclusions CCP is of value in the diagnosis and treatment of cancer patients.更多还原