【摘要】 目的:探讨特发性血小板减少性紫癜(ITP)患者骨髓血小板生成素(Tpo)和其受体c-mpl基因变化的意义。方法:用非放射性核素细胞原位杂交法检测tpo、c-mpl mRNA;细胞免疫化学法检测巨核细胞MPL。结果:ITP患者骨髓tpo、c-mpl mRNA,巨核细胞MPL表达增高。结论:c-mpl基因是在转录水平调控的;人体骨髓内对tpo及其受体的调节是以负反馈方式进行的;ITP患者骨髓局部tpo表达增高,病理情况下巨核细胞MPL增高可能与循环Tpo水平降低有关。更多还原

【Abstract】 Objective: To explore the expression of thrombopoietin (Tpo) and its receptor c-mpl in bone marrow from patients with idiopathic thrombocytopenic purpura(ITP). Methods: Non-radioactive in situ hy- bridization was used to detect tpo, c-mpl mRNA. Immune cytochemistry was used to detect MPL on megakaryocyte. Results: The expression of tpo, c-mpl mRNA in bone marrow and MPL on megakaryocyte in patients with ITP was increased. Conclusion: c-mpl mRNA was regulated at transcriptional level. A negative feedback loop was involved in the regulation of megakaryopoiesis in bone marrow. MPL may be involved in the regulation of circulating Tpo.更多还原