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哮喘患者外周血嗜酸性粒细胞抗体的表达及临床意义

The changes of EG1 and EG2 positive eosinophils and their clinical significance in asthmatics

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【作者】 黄宏徐军钟南山

【Author】 HuangHong, XuJun, Zhong Nanshan. Guangzhou Institute of Respiratory Disease,Guangzhou 510120

【机构】 广州呼吸疾病研究所

【摘要】 目的评价哮喘患者外周血EG1和EG2的表达及与哮喘严重程度的关系。方法采集68例哮喘患者、慢性阻塞性肺疾病(COPD)及健康人外周静脉血,经细胞膜穿透法(FOG)处理后用荧光标记,在流式细胞仪上进行分析。结果29例症状性哮喘患者外周血EG1的平均荧光强度(MFI)是1238±807,阳性细胞百分比是70%±19%;EG2的MFI为1002±658,阳性细胞百分比为79%±18%;13名健康对照组EG1的MFI为435±98,阳性细胞百分比为56%±22%;EG2的MFI为404±80,阳性细胞百分比为58%±22%;14例哮喘缓解期患者EG1的MFI为605±439,阳性细胞百分比为64%±19%,EG2的MFI为614±404,阳性细胞百分比为69%±16%;12例COPD急性发作期患者EG1的MFI为440±115,阳性细胞百分比为52%±16%,EG2的MFI为407±151,阳性细胞百分比为53%±14%;P<0.05。且与哮喘发作时的程度正相关:EG1的MFI与症状相关(r=0.70,P<001);EG2的MFI与症状相关(r=0.77,P<005)。结论EG1和EG2的表达,反应哮喘气道的严?

【Abstract】 Objective To evaluate the significance of EG1 or EG2 positive eosinophils in relating to the severity of asthma. Method EDTA anticoagulated venous blood were treated by FOG method, and FITC conjugated monoclonal antibodies were added. Flowcytometry technique was used. Result EG1 and EG2 positive eosinophils increased significantly in patients with acute asthma ( n =29) as compared with control ( n =13) and COPD ( n =12), P <0.05.There were significant correlation of the mean fluorescence intensity(MFI) between EG1,EG2 and the degree of the symptom. Conclusion There is a significant increase of activated eosinophils in peripheral blood in asthmatics, the expression of EG1 and EG2 positive eosinophils correlated well with the severity of symptom in asthmatics. EG1 and EG2,as the marker for eosinophils activity, may be sensitive and specific in evaluating the severity of asthmatics symptom.

【关键词】 哮喘嗜酸性粒细胞抗体
【Key words】 Asthma Eosinophil Antibody
  • 【文献出处】 中华结核和呼吸杂志 ,CHINESE JOURNAL OF TUBERCULOSIS AND RESPIRATORY DISEASES , 编辑部邮箱 ,1998年10期
  • 【分类号】R562.250.3
  • 【被引频次】35
  • 【下载频次】147
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