【摘要】 目的评价哮喘患者外周血ＥＧ１和ＥＧ２的表达及与哮喘严重程度的关系。方法采集６８例哮喘患者、慢性阻塞性肺疾病（ＣＯＰＤ）及健康人外周静脉血，经细胞膜穿透法（ＦＯＧ）处理后用荧光标记，在流式细胞仪上进行分析。结果２９例症状性哮喘患者外周血ＥＧ１的平均荧光强度（ＭＦＩ）是１２３８±８０７，阳性细胞百分比是７０％±１９％；ＥＧ２的ＭＦＩ为１００２±６５８，阳性细胞百分比为７９％±１８％；１３名健康对照组ＥＧ１的ＭＦＩ为４３５±９８，阳性细胞百分比为５６％±２２％；ＥＧ２的ＭＦＩ为４０４±８０，阳性细胞百分比为５８％±２２％；１４例哮喘缓解期患者ＥＧ１的ＭＦＩ为６０５±４３９，阳性细胞百分比为６４％±１９％，ＥＧ２的ＭＦＩ为６１４±４０４，阳性细胞百分比为６９％±１６％；１２例ＣＯＰＤ急性发作期患者ＥＧ１的ＭＦＩ为４４０±１１５，阳性细胞百分比为５２％±１６％，ＥＧ２的ＭＦＩ为４０７±１５１，阳性细胞百分比为５３％±１４％；Ｐ＜０．０５。且与哮喘发作时的程度正相关：ＥＧ１的ＭＦＩ与症状相关（ｒ＝０．７０，Ｐ＜００１）；ＥＧ２的ＭＦＩ与症状相关（ｒ＝０．７７，Ｐ＜００５）。结论ＥＧ１和ＥＧ２的表达，反应哮喘气道的严?更多还原

【Abstract】 Objective To evaluate the significance of EG1 or EG2 positive eosinophils in relating to the severity of asthma. Method EDTA anticoagulated venous blood were treated by FOG method, and FITC conjugated monoclonal antibodies were added. Flowcytometry technique was used. Result EG1 and EG2 positive eosinophils increased significantly in patients with acute asthma ( n =29) as compared with control ( n =13) and COPD ( n =12), P <0.05.There were significant correlation of the mean fluorescence intensity(MFI) between EG1,EG2 and the degree of the symptom. Conclusion There is a significant increase of activated eosinophils in peripheral blood in asthmatics, the expression of EG1 and EG2 positive eosinophils correlated well with the severity of symptom in asthmatics. EG1 and EG2,as the marker for eosinophils activity, may be sensitive and specific in evaluating the severity of asthmatics symptom.更多还原