【摘要】 目的研究低剂量辐射对Ⅰ型辅助性Ｔ细胞（Ｔｈ１）和Ⅱ型辅助性Ｔ细胞（Ｔｈ２）的影响，揭示低剂量辐射使Ｔｈ激活的分子机理。方法采用分子杂交方法，检测Ｘ射线全身照射后小鼠脾脏和胸腺细胞ＩＦＮ－γ和ＩＬ－６ｍＲＮＡ的表达。结果７５ｍＧｙＸ射线全身照射后小鼠脾脏和胸腺ＩＦＮ－γ和ＩＬ－６ｍＲＮＡ表达水平明显增高。结论低剂量辐射诱导Ｔｈ１和Ｔｈ２激活，其分子机理与其启动调节Ｔｈ１和Ｔｈ２克隆发育的细胞因子的基因表达有关更多还原

【Abstract】 Objective\ To elucidate the possible mechanism of activation of helper T cells after low dose radiation (LDR) exposure,the influence of whole body irradiation (WBI) with 75mGy X rays on Th1 and Th2 was studied. Methods\ Mice were irradiated at a dose rate of 12 5mGy/min,and IFN γ and IL 6 mRNA levels of the mocytes and splenocytes were analyzed by dot blot and Northern blot hybridization.Results\ It was found that IFN γ and IL 6 mRNA significantly increased after WBI with 75mGyX rays.Conclusion\ The gene induction of cytokine profile after LDR indicates that activation of both Th1 and Th2 subtypes may be involved in the stimulation of immune functions by LDR.更多还原