【摘要】 目的从细胞凋亡探讨不同年龄妇女卵母细胞的质量差异。方法应用细胞培养技术，和原位ＤＮＡ片段末端标记（ＩＳＥＬ）方法，对５８例卵巢囊肿、畸胎瘤患者的２０６个卵母细胞按不同年龄组进行观察和研究。结果卵母细胞的凋亡过程与其它细胞一样，开始细胞容积缩小，形态不规则，最后凋亡小体形成等；４１～５０岁年龄组妇女卵母细胞凋亡较２１～３０岁与３１～４０岁年龄组进展快（凋亡过程时间短，Ｐ＜０．００５）及凋亡率高（Ｐ＜０．０１）。结论４１～４５岁年龄组卵母细胞质量差及对促超排卵反应低的原因，可能是在此年龄组中有较多的卵母细胞发生凋亡更多还原

【Abstract】 Objective To study the morphological characteristics, and rate of apoptosis of human oocytes in relation to age. Methods 206 oocytes were aspirated from antral follicles 4～6 mm in diameter from 58 patients who had surgery done for ovarian cysts. These oocytes were incubated in Ham’s F10 with 10% fetal cord serum (FCS) for 48～120 hours, and examined under inverted microscope every 6～8 hours to see their morphological changes. In situ endlabeling (ISEL) of fragmented DNA was used to confirm apoptosis. Results The characteristics of apoptosis are:(1)shape change of oocytes. (2) oocyte shrinkage. (3) chromatic condensation and apoptotic body formation. (4) internucleosomal DNA cleavage was shown by ISEL. Apoptosis occurred in 13.9% (women aged 21～30), 37.7% (women aged 31～40), and 61.5% (women aged 41～50) respectively. The rate of apoptosis was much higher in women aged 41～50 than that in the other two groups. Conclusion The rate of apoptosis of human immature oocytes cultured in vitro is much higher in older women aged 41～50 than in those aged 21～40, which may account for the poor response to superovulation stimulation and low oocyte quality in older women.更多还原