【摘要】 目的评价干扰素α（ＩＦＮα）、肿瘤坏死因子α（ＴＮＦα）对白细胞介素２（ＩＬ２）活化的脐血体外杀伤白血病细胞株作用的影响。方法采用３ＨＴｄＲ掺入法和半固体培养法。结果三种细胞因子中仅ＩＬ２可活化脐血产生对体外培养的白血病细胞株的杀伤作用；ＩＦＮα、ＴＮＦα与ＩＬ２联合应用，可产生协同效应，其杀伤率明显高于单用ＩＬ２活化组４到１０个百分点；ＩＬ２单独或联合ＩＮＦα，不影响脐血造血祖细胞的活性（ＰＣＡ），ＴＮＦα单独或联合ＩＬ２应用则对ＰＣＡ有明显抑制作用，抑制程度在６０％左右。结论ＩＮＦα、ＴＮＦα均能协同ＩＬ２活化脐血，提高活化脐血的抗肿瘤活性；ＩＦＮα联合ＩＬ２不影响脐血ＰＣＡ。更多还原

【Abstract】 Objective To evaluate the influence of interferon α(IFN α) and TNF α on the killing effect of interleukin 2 (IL 2) activated cord blood on CEM and K562 cell strains. Methods TdR release assay and semisolid culture method were used in this study. Results Among the three cytokines, only IL 2 was able to activate cord blood to generate anti tumor activity. IFN α and TNF α played the synergetic role with IL 2 during activation. Coculturing with either of the two cytokines, the killing ratio of activated cord blood was significantly improved by 4 to 10 percent. IL 2 alone or cooperated with IFN α did not inhibit progenitor cell activity (PCA) of activated cord blood, but TNF α alone or cooperated with IL 2 did. The inhibition degree was about 60%. Conclusion TNF α and IFN α could cooperate with IL 2 to activate cord blood to kill leukemia cells. Using IL 2 together with IFN α for activation did not inhibit cord blood PCA, which could benefit hematopoiesis reconstitution.更多还原