【摘要】 目的探索阻断肿瘤转移新的途径。方法利用内源性层粘连蛋白（ＬＮ）及其受体（ＬＮＲ）强阳性的小鼠纤维样肉瘤细胞，分别在体外经外源性ＬＮ、蛋白激酶Ｃ（ＰＫＣ）抑制剂（Ｓｔａｕｒｏｓｐｏｒｉｎｅ，ＳＰ）、抗ＬＮＲ、抗ＬＮ处理后，一次性注入Ｃ５７小鼠尾静脉（７７只），使之在肺内成瘤。各组瘤细胞在未注入前，均经流式细胞仪（ＦＣＭ）定量测定其表面ＬＮＲ水平。结果空白对照组、ＬＮ组、ＳＰ组、抗ＬＮＲ组、抗ＬＮ组，瘤细胞表面ＬＮＲ阳性率顺次为５６５％、７６３％、５０２％、３１９％、２０６％，小鼠肺内瘤灶（含原发及转移灶）体积均值顺次为７６ｍｍ３／只，１１０ｍｍ３／只，１４ｍｍ３／只，５ｍｍ３／只，２０ｍｍ３／只，小鼠肺内淋巴管血管腔内瘤栓形成率顺次为８１％、９４％、３６％、４３％、６８％。结论外源性ＬＮ组的病变及转移程度重于空白对照组，而其余各组均轻于空白对照组；统计学分析表明ＬＮＲ水平与瘤灶大小及瘤栓形成率（病变程度及转移程度）均呈正相关；体内瘤细胞内磷脂肌醇传导系统的功能状态亦与之密切相关。更多还原

【Abstract】 Objective To study methods for blocking tumor metastasis. Methods Endogenous laminin (LN), protein kinase C (PKC) inhibitor staurosporine (SP), anti LN recetpr (LN R) were used to act on murine fibrosarcoma cells which were strongly positive for endogenous LN and LN R, then infused into the caudal veins of 77 C 57 mice once, to initiate lung tumor formation. Before infusion, the cells were all measured for surface LN R quantity with flow cytometry. Results The positive rate of LN R on tumor cell surface in the control group, LN group, SP group, anti LN R group and anti LN group being 56.5%, 76.3%, 50.2%, 31.9% and 20.6% respectively. The average volume of the tumors in the lungs of mice being 76 mm 3, 110 mm 3, 14 mm 3, 5 mm 3 and 20 mm 3 respectively. The rate of tumor embolus formation in lymphatic and blood vessels being 81%, 94%, 36%, 43% and 68% respectively. Conclusion The pathological changes and metastasis in the LN group are more svere than that in control group. But all other groups are less severe than the control group. The level of LN R on tumor cell surface is positively correlated with tumor volume and rat of tumor embolus formation. The lipositol conduction system in tumor cells is also closerly related with size of tumor foci and rate of tumor embolus formation.更多还原