【摘要】 海藻硫酸多糖(SP)是一种从褐藻中提取的天然硫酸酯化多糖,它具有多种药理作用。国内外对其辐射防护作用的研究仅限于整体水平。该课题就动物整体、细胞及分子水平对SP的辐射防护作用及其可能机理进行了研究:(1)以γ射线照射小鼠为模型,观察了SP对受照小鼠的外周血恢复及30d存活率的影响;(2)以造血因子依赖细胞株(NFS—60及TF—1)为靶细胞,采用MTT比色法建立了简便且重复性好的体外照射模型,研究了SP对造血细胞的辐射防护作用;(3)应用ESR技术,以5′—TMP作为DNA模型,研究了SP清除辐射所致自由基的作用。结果发现,SP确有促进受照小鼠外周血恢复,促进造血细胞增殖,提高其30d存活率和清除自由基的作用。更多还原

【Abstract】 Seaweed sulfated polysaccharide (SP) is a natural sulfated polysaccharide isolated from seaweed and has various pharmacological activities. However, previous studies on the radioprotective effect of SP were limited to in vivo whole-body studies. In the present study, SP was examined for radioprotective effect and its mechanism by MTT assay and the electron spin resonance (ESR). The items studied were as follows : (1) Different doses of SP were given to 60Co r-ray irradiated KM mice,and their influence on the peripheral blood recovery and 30 d survival rate of the irradiated mice were observed. (2) The radioprotective mechanism of SP on hematopoietic cells was studied in two hematopoietic growth factor-dependent cell lines (NFS-60 to G-CSF,TF-1 to GM-CSF) by MTT assay. (3) The irradiated 5’-TMP was used as a model for estimating the amount of DNA to observe the protective activity of SP on the radiation injury of DNA by using ESR. The results found that SP could promote the recovery of peripheral blood and proliferation of the hematopoietic cells in irradiated mice,increase their 30 d survival rate,and scavenge the free radicals.更多还原