【摘要】 目的：建立地黄中腺苷含量的测定方法，测定不同品种地黄中腺苷的含量。方法：采用高效液相色谱法测定地黄中腺苷含量。以ＫＹＷＧＣ１８为固定相，６％乙腈为流动相，检测波长为２６０ｎｍ。结果：腺苷在０．００２ｍｇ／ｍｌ～０．０１ｍｇ／ｍｌ线性良好。回归方程为Ｙ＝１．７７４２×１０－４＋８．９０２１×１０－７Ｘ，ｒ＝０．９９９５。平均回收率为９４．１％。ＲＳＤ＝１．８６％（ｎ＝５）。结论：本法简便、快速、结果可靠。更多还原

【Abstract】 Objective:To establish a method to determine the contents of asenosine in different species of Rehmannia glutinosa.Method:The contents were determined by HPLC,with a colunm of KYWG C 18 as stationary phase,6% ace tonitrile as mobile phase and detecting wavelength at 260nm.Result:Adenosine has a good linearity in the range of 0.002mg/ml～0.01mg/ml,Y=1.7742×10 -4 +8.9021×10 -7 X,r=0.9995.The average recovery is 94.1% and RSD=1.86% (n=5).Conclusion:The method is fast,reliable and simple.更多还原