【摘要】 用Ｓｏｕｔｈｅｒｎ分析法检测了２５例乳腺癌组织中ｃ－ｍｙｃ基因的拷贝数及多态性，发现其中１２例（４８％）有ｃ－ｍｙｃ基因的扩增，长度分别为１３ｋｂ和２３ｋｂ。高龄患者中（≥４５岁），ｃ－ｍｙｃ扩增的阳性率（１１／１６，６８％）高于成年患者（＜４５岁）（１／９，１０％，Ｐ＜０．０５），同时证实，腋窝淋巴结转移出现ｃ－ｍｙｃ扩增的比例（１２／１７，７０％）明显高于无腋窝淋巴结转移者（１／８，１２％，Ｐ＜０．０５）。结果表明，ｃ－ｍｙｃ基因扩增与乳腺癌腋窝淋巴结转移及高龄状态发病有密切关系。我们认为，ｃ－ｍｙｃ经扩增方式激活在更年期女性乳腺癌发生过程中可能起着重要的作用。更多还原

【Abstract】 Using Southern blot assay we examined the amplification of 13kb and/or 23kb c myc oncogen in human breast cancer. Of 25 patients tested, 48%(12/25)showed po sitive reaction. The positive rate was higher in the aged group (≥46 years) (68%,11/16) than that in the adult group (≤45 years) (10%, 1/9), P <0.05.Higher positive incidence was also found in breast cancer with axillary lymph node involvement (70%, 12/17) than in that without lymph node involvement (12%, 1/8) ( P <0.05). Our results showed that c myc may play variable roles in adult and aged patients with breast cancer. It is concluded that c myc amplification may reflect the proliferation activity and invasiveness of tumor cells and that it can be used as an independent factor for evaluating the prognosis of climacteric patients.更多还原