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TLC测定冬凌草叶中冬凌草甲、乙素的含量

TLC in quantitative determination of oridonin and ponicidin in the leaves of Rabdosia rubescens

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【作者】 袁珂胡润淮杨怡孙伟张晓明

【Author】 Yuan Ke (Yuan K), Hu Runhuai (Hu RH), Yang Yi (Yang Y), et al (He’nan College of Traditional Chinese Medicine, Zhengzhou 450003)

【机构】 河南中医学院中药系

【摘要】 目的:研究冬凌草叶中冬凌草甲、乙素的含量测定。方法:采用薄层扫描法进行测定。结果:冬凌草甲素浓度在9.50μg~47.50μg之间线性关系良好,相关系数r=0.999,回收率为98.54%,测定的RSD=1.41%。冬凌草乙素浓度在9.98μg~49.90μg之间线性关系良好,相关系数r=0.999,回收率为98.07%,测定的RSD=1.35%。结论:本法简便、准确、灵敏,重现性好,可用于冬凌草的质量控制。由本法测定结果表明,生长在济源太行山区8月份的冬凌草叶中冬凌草甲、乙素的含量最高

【Abstract】 OBJECTIVE: To study the method for quantitative determination of oridonin and ponicidin in the leaves of Rabdosia rubescens. METHODS: TLCS method was selected to determine the content. RESULTS: For oridonin the linear range was 9.50 μg~47.50 μg with r=0.999, the average recovery was 98.54% with RSD 1.41%. For ponicidin the linear range was 9.98 μg~49.90 μg with r=0.999, the average recovery was 98.07% with RSD 1.35%. CONCLUSION: The method was proved to be simple, precise sensitive and reproduciable. It can be used for the quality control of Rabdosia rubescens. The results showed that the content of oridonin and ponicidin in the leaves of Rabdosia rubescens, which grows in August in Ji Yuan county area, is relatively the highest.

【基金】 河南省自然科学基金
  • 【文献出处】 中国药学杂志 ,CHINESE PHARMACEUTICAL JOURNAL , 编辑部邮箱 ,1998年10期
  • 【分类号】R284.1
  • 【被引频次】30
  • 【下载频次】198
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