【摘要】 建立了分光光度法测定人血清中β－胡萝卜素的浓度。样品用正己烷提取后，在450nm处测定。β－胡萝卜素的线性范围0．25～4μg／ml，最低检出浓度0．0625μg／ml，平均回收率96．11±3．09％（n＝9）。应用本法对12名健康志愿者自体交叉口服不同厂家的β－胡萝卜素片剂和胶囊剂后的血药浓度进行了测定，结果表明，2种制剂的t1／2Ke分别为4．33d和4．82d，各个体AUC（0～22d）的均值分别为18．51和16．13μg／（ml·d），β－胡萝卜素片剂的相对生物利用度为114．76％，经统计分析，2种制剂具有生物等效性。更多还原

【Abstract】 An UV-spectrophotometry has been developed for determination of β-carotene in serum. β-carotene in samples wasextracted with n-hexane,detected at UV450nm.There was a good linear relationship within the range of 0.25-4μg/ml.The detactableminimum concentration this drug in serum was 0.0 625μg/ml.The average recovery was 96.11 ±3.09%（n=9）.This method has beenapplied to monitoring serum levels of β-carotene in 12 adult male volunteers who were divided into two groups and receivedalternative p.o.administration of either H β-carotene tablets or S β-carotene capsules.The results showed that t₁-2 Ke and AUC₀-22d oftwo forms of β-carotene preparation were 4.33d and 4.82d;18.51 and 16.13μg/（ml. d）,res pectively.The relative bioavailability of Hβ-carotene tablet was 114.76% .The results showed that the two forms of drug were bioequialent.更多还原