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T-2毒素致DNA损伤研究

Study on DNA damage caused by T2 toxin

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【作者】 刘宁任志鸿

【Author】 Liu Ning,Ren ZhihongKashinBeck Institute,Chinese Research Center for Endemic Disease Control,Harbin 150001

【机构】 中国地方病防治研究中心大骨节病研究所

【摘要】 新生肉鸡鸡雏经口给予不同剂量的T-2毒素,一周后处死,常规酚抽提法提取肝、心、肾、肌肉及软骨组织的DNA,1.8%琼脂糖凝胶电泳观察。结果显示,T-2毒素可致多种组织DNA损伤,但程度不同。大剂量以细小碎片为主,降低剂量,大碎片增多,且可见有规则的断裂带发生。对实验结果,做了扼要讨论。

【Abstract】  Different doses of T2 toxin were fed to newborn chickens.Samples from liver,heart,kidney,muscle and cartilage were collected after one week.DNA was extracted by a routine method.Electrophoresis of DNA was carried out in 1.8% of agarose gel and DNA damage patterns were observed on a ultroviolet transilluminator.The result showed that DNA damage could be caused by T2 toxin in many tissues,but the extent was different.DNA was cleaned into a lot of small fragments when a higher dose was given.Big fragments increased with the doses decreased.Regular DNA fragmentation band was found under the circumstance of the lower dose of T2 toxin.

  • 【文献出处】 中国地方病学杂志 ,CHINESE JOURANL OF CNDEMIOLOGY , 编辑部邮箱 ,1998年02期
  • 【分类号】S852.44,Q523
  • 【被引频次】14
  • 【下载频次】131
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