【摘要】 目的和方法：采用体外细胞培养，进行氚标胸腺嘧啶核苷（３Ｈ－ＴｄＲ）掺入和原位杂交。结果：发现γ－干扰素（ＩＦＮ－γ）能抑制体外培养新西兰兔血管平滑肌细胞（ＶＳＭＣ）的ＤＮＡ合成和血小板衍化生长因子Ａ链（ＰＤＧＦ－Ａ）的基因表达；整体实验中，用４ＦＦｏｇａｒｔｙ导管气囊拉伤新西兰兔的腹主动脉内皮后，随即连续肌注ＩＦＮ－γ（１６５万Ｕ·ｋｇ１·ｄ１）３天，结果发现肌注ＩＦＮ－γ能明显抑制受损部位ＶＳＭＣ的３Ｈ－ＴｄＲ的标记率及ＰＤＧＦ－Ａ的表达。结论：ＩＦＮ－γ对血管成形术引起的血管挛缩有一定的舒张作用。更多还原

【Abstract】 AIM and METHODS: 3 H-thymidine incoporation and in situ hybridization with PDGF-A cDNA probe were performed in cultured smooth muscle cells and restenosis model after angioplasty.RESULTS:Interferon-γ inhibited vascular smooth muscle cell DNA synthesis and the expression of PDGF-A. In vitro experiment showed that intramuscular injection of interferon-γ for three days decreased the labeling rate of 3 H thymidine and the mRNA of PDGF-A.CONCLUSION:The interferon-γ diminished the vascular constriction induced by injury, and had some vascular relaxation effects.更多还原