【摘要】 目的和方法：Ｈｅｙｍａｎｎ肾炎（ＨＮ）是一种用于研究人类膜性肾病的大鼠动物模型。用主动型ＨＮ模型大鼠的淋巴细胞与小鼠ＳＰ２／０骨髓瘤细胞融合，制备针对ＨＮ致病原的单克隆抗体ｃ３～６（ＭｃＡｂｃ３～６），并用它诱导大鼠被动型ＨＮ。结果：免疫酶组织化学及免疫电镜显示，ＭｃＡｂｃ３～６特异性识别肾小球上皮足突细胞膜抗原并与其结合形成免疫复合物。更多还原

【Abstract】 AIM and METHOD:Heymann glomerulonephritis in rats is a typical model of human membranous nephropathyThe lymphocytes of Heymann glomerulonephritis rat were fused with a mouse SP 2/0 myeloma cell lineRESULTS:One rat /mouse hybridoma c3～6 was produced and proved to be positive by ELISA When the monoclonal antibody c3～6 was injected intravenously into rats, immune deposits were observed in the glomerular capillary by direct immunoperoxidase 5 days, later.Further investigation by direct immunoelectron microscopy showed that the immune deposits were located between the podocyte and the glomerular basement membrane. CONCLUSION:Circulating antibody may bind to podocyte surface or formation of immune complexes in situ in the glomeruli and result in the initiation of Heymann glomerulonephritis. .更多还原