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一株产生EB病毒的狨猴淋巴细胞株的建立

Establishment of a Marmoset Lymphoblastoid Cell Line Releasing Transforming Epstein Barr Virus

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【作者】 孙晓梅; 梁梧生; 代解杰; 高家鸿;

【Author】 Sun Xiaomei\ \ Liang Wusheng\ \ Dai Jiejie\ \ Gao Jiahong (Chinese Academy of Medical Sciences, Peking Union Medical College, Institute of Medical Biology, Kunming 650107)

【机构】 中国协和医科大学医学生物学研究所;

【摘要】 用B958细胞株产生的EB病毒,转化棉顶狨猴外周血淋巴细胞,获得KMT3细胞株。该细胞株能产生高滴度的EB病毒。含EB病毒衣壳抗原的自然阳性细胞率为3~5%,激活后达50~60%,其培养上清液可直接转化人淋巴细胞得到传代细胞系。KMT3细胞染色体数2n=46条

【Abstract】 A lymphoblastoid cell line (KMT 3) was established by EBV induced B cell transformation in the cotton top tamarin. The viruses were prepared from the culture fluid of B 95 8 cells by passing through a 0.45μm filter. KMT 3 can produce high titer infections EB viruses. Its VCA positive cell rate was 3~5% in natural and 50~60% after activation. Human B lymphocytes could be immortalized directly from KMT 3 supernatant. The number of chromosomes was 46.

  • 【文献出处】 中国病毒学 ,VIROLOGICA SINICA , 编辑部邮箱 ,1998年01期
  • 【分类号】Q939.4,
  • 【下载频次】41
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